Knee extension and hip flexion range of motion (ROM) and functional performance of the hamstrings are of great importance in many sports. The aim of this study was to investigate if static stretching (SS) or vibration foam rolling (VFR) induce greater changes in ROM, functional performance, and stiffness of the hamstring muscles. Twenty-five male volunteers were tested on two appointments and were randomly assigned either to a 2 min bout of SS or VFR. ROM, counter movement jump (CMJ) height, maximum voluntary isometric contraction (MVIC) peak torque, passive resistive torque (PRT), and shear modulus of semitendinosus (ST), semimembranosus (SM), and biceps femoris (BF_lh), were assessed before and after the intervention. In both groups ROM increased (SS = 7.7%, P < 0.01; VFR = 8.8%, P < 0.01). The MVIC values decreased after SS (-5.1%, P < 0.01) only. Shear modulus of the ST changed for -6.7% in both groups (VFR: P < 0.01; SS: P < 0.01). Shear modulus decreased in SM after VFR (-6.5%; P = 0.03) and no changes were observed in the BF_lh in any group (VFR = -1%; SS = -2.9%). PRT and CMJ values did not change following any interventions. Our findings suggest that VFR might be a favorable warm-up routine if the goal is to acutely increase ROM without compromising functional performance.

• Both interventions (vibration foam rolling and static stretching) lead to the similar increase in hip flexion range of motion.

• Vibration foam rolling of the hamstrings reduces muscle stiffness of the Semitendinosus (ST) and Semimembranosus (SM) muscles, while static stretching lead to a reduction in muscle shear modulus of the ST only.

• Maximal voluntary isometric contraction peak torque is reduced after prolonged (2 min) static stretching but not after a similar duration of vibration foam rolling.